Recombinant Human DNMT1 protein, His & T7-tagged

• Cat.No.: DNMT1-1645H
• Product Overview: Recombinant Human DNMT1 aa. (Val1343~Leu1570 (Accession # P26358)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 29.6kDa
• Protein length: Val1343~Leu1570
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 9.7

Gene Information

• Gene Name: DNMT1 DNA methyltransferase 1 [ Homo sapiens (human) ]
• Official Symbol: DNMT1
• Synonyms: DNMT1; DNA methyltransferase 1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; m.HsaI; DNA (cytosine-5)-methyltransferase 1; CXXC-type zinc finger protein 9; DNA (cytosine-5-)-methyltransferase 1; DNA MTase HsaI; DNA methyltransferase HsaI
• Gene ID: 1786
• mRNA Refseq: NM_001130823.2
• Protein Refseq: NP_001124295.1
• UniProt ID: P26358