Recombinant Human DNMT1, His-tagged

• Cat.No.: DNMT1-388H
• Product Overview: The enzymes are His-tagged and overexpressed in Baculovirus. Lysates are produced by sonication in the presence of protease inhibitors and the proteins affinity purified over nickel-agarose columns (eluted with imidazole buffer).

Specification

• Description: DNA methylation at CpG sites is an essential feature of gene regulation in higher eukarya. Methylation of cytosine residues is correlated with silenced chromatin and there is strong evidence that inappropriate methylation can disrupt normal growth control circuits. The process is mediated by enzymes called DNA methyltransferases (DNMTs) and a number of different DNMT isoforms have been identified, cloned and characterized. DNMT isoforms fall into two broad groupings: 1) Maintenance activities which preferentially act on hemi-methylated DNA, and 2) De novo activities which initiate methylation on a unmethylated DNA targets. DNMT1 is a maintenance enzyme that most likely targets replicative or nascent DNA. DNMT3 type isoforms appear to be de novo methyltransferases. Other DNMTs are now known to be active but have not been classified (DNMT2) while still other isoforms appear to possess key motifs of DNMT but lack sequences necessary for catalytic activity (DNMT3L).
• Source: Baculovirus
• Species: Human
• Tag: His
• Storage: The enzymes are shipped on Dry Ice. Detailed temperature stabilization time profiles have not been performed; however, store at -20oC to -70oC and avoid repeat freeze thawing cycles (it is best to aliquot the enzyme on first thaw). The enzymes are stable for 6 months from date of shipping under these conditions.

Gene Information

• Gene Name: DNMT1 DNA (cytosine-5-)-methyltransferase 1 [ Homo sapiens ]
• Official Symbol: DNMT1
• Synonyms: DNMT1; DNA (cytosine-5-)-methyltransferase 1; DNMT; DNA (cytosine-5)-methyltransferase 1; CXXC9; MCMT; m.HsaI; DNA MTase HsaI; CXXC finger protein 9; DNA methyltransferase 1; DNA methyltransferase HsaI; CXXC-type zinc finger protein 9; AIM; HSN1E; FLJ16293; MGC104992
• Gene ID: 1786
• mRNA Refseq: NM_001130823
• Protein Refseq: NP_001124295
• MIM: 126375
• UniProt ID: P26358
• Chromosome Location: 19p13.2
• Pathway: Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Methionine degradation, organism-specific biosystem; Methionine degradation, conserved biosystem; One Carbon Metabolism, organism-specific biosystem; Regulation of retinoblastoma protein, organism-specific biosystem
• Function: DNA (cytosine-5-)-methyltransferase activity; DNA binding; DNA-methyltransferase activity; metal ion binding; protein binding; transcription factor binding; transferase activity; zinc ion binding