Recombinant Human DnaJ (Hsp40) Homolog, Subfamily B, Member 1, His-tagged;

• Cat.No.: DNAJB1-323H
• Product Overview: Recombinant human HSP40, his-tagged, is overexpressed in E.coli and purified by using the conventional column chromatography techniques (360 aa,40.2 kDa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Human HSP40 (heat shock protein with molecular size of 40kDa) is one of the mammalian homologues of bacterial DnaJ heat shock protein and regulates cellular processes by aiding in the folding, transport and assembly. HSP40 protein contains the J-domain which controls interaction with the ATPase domain of DnaK.
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 2 mM DTT, 0.2 mM PMSF, 10% glycerol.
• Molecular Weight: 40.2 kDa (360 aa).
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method).
• Sequence: MGSSHHHHHH SSGLVPRGSH MGKDYYQTLG LARGASDEEI KRAYRRQALR YHPDKNKEPG AEEKFKEIAE AYDVLSDPRK REIFDRYGEE GLKGSGPSGG SGGGANGTSF SYTFHGDPHA MFAEFFGGRN PFDTFFGQRN GEEGMDIDDP FSGFPMGMGG FTNVNFGRSR SAQEPARKKQ DPPVTHDLRV SLEEIYSGCT KKMKISHKRL NPDGKSIRNE DKILTIEVKK GWKEGTKITF PKEGDQTSNN IPADIVFVLK DKPHNIFKRD GSDVIYPARI SLREALCGCT VNVPTLDGRT IPVVFKDVIR PGMRRKVPGE GLPLPKTPEK RGDLIIEFEV IFPERIPQTS RTVLEQVLPI
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: DNAJB1
• Synonyms: DNAJB1; DnaJ (Hsp40) homolog, subfamily B, member 1; Hdj1; Sis1; HSPF1; Hsp40; DNAJ1; HDJ-1; HDJ1; DnaJ protein homolog 1; Heat shock protein 40; Heat shock 40 kDa protein 1; heat shock 40kD protein 1; DnaJ (Hsp40) homolog, subfamily B, member 1; Hsp40 (heat shock protein 40 kD).
• Gene ID: 3337
• mRNA Refseq: NM_006145.1
• Protein Refseq: NP_006136.1
• MIM: 604572
• UniProt ID: P25685
• Chromosome Location: 19p13.2
• Function: heat shock protein binding; unfolded protein binding.