Recombinant Human DNA topoisomerase II binding protein 1 Protein, His tagged

• Cat.No.: TOPBP1-100H
• Product Overview: Recombinant human TOPBP1 protein (Q92547) (Thr 1259-His 1522) with a His tag at the C-terminus was expressed in E. coli.

Specification

• Description: Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR.
• Source: E. coli
• Species: Human
• Tag: C-His
• Protein length: 1259-1522aa
• Molecular Mass: 31.6 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: TOPBP1 DNA topoisomerase II binding protein 1 [ Homo sapiens (human) ]
• Official Symbol: TOPBP1
• Synonyms: TOPBP1; topoisomerase (DNA) II binding protein 1; DNA topoisomerase 2-binding protein 1; KIAA0259; TOP2BP1; DNA topoisomerase II-binding protein 1; DNA topoisomerase II-beta-binding protein 1
• Gene ID: 11073
• mRNA Refseq: NM_007027
• Protein Refseq: NP_008958
• MIM: 607760
• UniProt ID: Q92547