Recombinant Human DNA polymerase kappa

• Cat.No.: POLK-137H
• Product Overview: This product was over-expressed as a recombinant protein in E. coli with a plasmid carrying a C-terminal histidine-tagged human DNA polymerase κ (1-560 aa), and highly purified by several steps of chromatography. The product is catalytically active and its molecular weight is 65 kD. Activity of this product has been confirmed by a user researcher even if it was diluted 8,000-fold.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-560 a.a.
• Description: Mammalian DNA polymerase κ, a member of the UmuC/DinB nucleotidyl transferase superfamily, has been implicated in spontaneous mutagenesis. Human DNA polymerase κ copies undamaged DNA with average single-base subsitution and deletion error rates of 7 x 10^-3 and 2 x 10^-3, respectively. These error rates are high when compared to those of most other DNA polymerases. DNA polymerase κ has important role in the mutagenic bypass of certain types of DNA lesions.
• Form: 0.2 M NaCl, 10 mM sodium phosphate buffer (pH 7.0), 50% glycerol
• Purity: Over 90% by SDS-PAGE (CBB staining)
• Applications: 1) Functional studies on translesion DNA synthesis by DNA polymerase kappa 2) Antigen for western blotting and ELISA by anti-Pol kappa antibody
• Storage: Sent at 4℃ or at -20℃. Upon arrival, centrifuge briefly and store at -20℃ or at -80℃ for longer storage.
• Concentration: 3.2 mg/ml as measured by BCA method

Gene Information

• Gene Name: POLK polymerase (DNA directed) kappa [ Homo sapiens ]
• Official Symbol: POLK
• Synonyms: POLK; polymerase (DNA directed) kappa; DINB1; DNA polymerase kappa; DINB protein; DINP; POLQ
• Gene ID: 51426
• mRNA Refseq: NM_016218
• Protein Refseq: NP_057302
• MIM: 605650
• UniProt ID: Q9UBT6
• Chromosome Location: 5q13
• Pathway: Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem
• Function: DNA-directed DNA polymerase activity; damaged DNA binding; metal ion binding; nucleotidyltransferase activity; transferase activity