Recombinant Human DMPK protein, His & T7-tagged

• Cat.No.: DMPK-7977H
• Product Overview: Recombinant Human DMPK aa. (Met1~Ile201 (Accession # Q09013)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Met1~Ile201
• Description: The protein encoded by this gene is a serine-threonine kinase that is closely related to other kinases that interact with members of the Rho family of small GTPases. Substrates for this enzyme include myogenin, the beta-subunit of the L-type calcium channels, and phospholemman. The 3' untranslated region of this gene contains 5-38 copies of a CTG trinucleotide repeat. Expansion of this unstable motif to 50-5,000 copies causes myotonic dystrophy type I, which increases in severity with increasing repeat element copy number. Repeat expansion is associated with condensation of local chromatin structure that disrupts the expression of genes in this region. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 27.1kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.7.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: DMPK DM1 protein kinase [ Homo sapiens (human) ]
• Official Symbol: DMPK
• Synonyms: DM; DM1; DMK; MDPK; DM1PK; MT-PK; DM protein kinase; dystrophia myotonica protein kinase; myotonic dystrophy associated protein kinase; myotonin protein kinase A; thymopoietin homolog; myotonin-protein kinase
• Gene ID: 1760
• mRNA Refseq: NM_001081560.2
• Protein Refseq: NP_001075029.1
• UniProt ID: Q09013