Recombinant Human DMBT1 protein(Met1-Ser220), His-tagged

• Cat.No.: DMBT1-3231H
• Product Overview: Recombinant Human DMBT1 (NP_004397.2) (Met 1-Ser 220) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-220 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized recombinant human Galectin-3 at 10 μg/ml (100 μl/well) can bind biotinylated DMBT1-His with a linear range of 0.06-1.0 μg/ml.
• Molecular Mass: The recombinant human DMBT1 consists of 211 amino acids and has a predicted molecular mass of 22.6 kDa. The apparent molecular mass of rh DMBT1 is approximately 35-45 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: DMBT1 deleted in malignant brain tumors 1 [ Homo sapiens ]
• Official Symbol: DMBT1
• Synonyms: DMBT1; deleted in malignant brain tumors 1; deleted in malignant brain tumors 1 protein; GP340; muclin; SAG; gp-340; hensin; glycoprotein 340; salivary agglutinin; surfactant pulmonary-associated D-binding protein; FLJ61058; MGC164738
• Gene ID: 1755
• mRNA Refseq: NM_004406
• Protein Refseq: NP_004397
• MIM: 601969
• UniProt ID: Q9UGM3