Recombinant Human DIAPH1 protein, His & GST-tagged

• Cat.No.: DIAPH1-1649H
• Product Overview: Recombinant Human DIAPH1 aa. (Asp389~Pro583 (Accession # O60610)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: Asp389~Pro583
• Description: This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 54.5kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 5.7

Gene Information

• Gene Name: DIAPH1 diaphanous related formin 1 [ Homo sapiens (human) ]
• Official Symbol: DIAPH1
• Synonyms: DIAPH1; diaphanous related formin 1; DIA1; DRF1; DFNA1; LFHL1; SCBMS; hDIA1; protein diaphanous homolog 1
• Gene ID: 1729
• mRNA Refseq: NM_001079812.2
• Protein Refseq: NP_001073280.1
• UniProt ID: O60610