Recombinant Human DHX30 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: DHX30-4118H
• Product Overview: DHX30 MS Standard C13 and N15-labeled recombinant protein (NP_055781) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The family member encoded by this gene is a mitochondrial nucleoid protein that associates with mitochondrial DNA. It has also been identified as a component of a transcriptional repressor complex that functions in retinal development, and it is required to optimize the function of the zinc-finger antiviral protein. Alternatively spliced transcript variants have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 129.4 kDa
• AA Sequence: MAASRDLLKEFPQPKNLLNSVIGRA LGISHAKDKLVYVHTNGPKKKKVTL HIKWPKSVEVEGYGSKKIDAERQAA AAACQLFKGWGLLGPRNELFDAAKY RVLADRFGSPADSWWRPEPTMPPTS WRQLNPESIRPGGPGGLSRSLGREE EEDEEEELEEGTIDVTDFLSMTQQD SHAPLRDSRGSSFEMTDDDSAIRAL TQFPLPKNLLAKVIQIATSSSTAKN LMQFHTVGTKTKLSTLTLLWPCPMT FVAKGRRKAEAENKAAALACKKLKS LGLVDRNNEPLTHAMYNLASLRELG ETQRRPCTIQVPEPILRKIETFLNH YPVESSWIAPELRLQSDDILPLGKD SGPLSDPITGKPYVPLLEAEEVRLS QSLLELWRRRGPVWQEAPQLPVDPH RDTILNAIEQHPVVVISGDTGCGKT TRIPQLLLERYVTEGRGARCNVIIT QPRRISAVSVAQRVSHELGPSLRRN VGFQVRLESKPPSRGGALLFCTVGI LLRKLQSNPSLEGVSHVIVDEVHER DVNTDFLLILLKGLQRLNPALRLVL MSATGDNERFSRYFGGCPVIKVPGF MYPVKEHYLEDILAKLGKHQYLHRH RHHESEDECALDLDLVTDLVLHIDA RGEPGGILCFLPGWQEIKGVQQRLQ EALGMHESKYLILPVHSNIPMMDQK AIFQQPPVGVRKIVLATNIAETSIT INDIVHVVDSGLHKEERYDLKTKVS CLETVWVSRANVIQRRGRAGRCQSG FAYHLFPRSRLEKMVPFQVPEILRT PLENLVLQAKIHMPEKTAVEFLSKA VDSPNIKAVDEAVILLQEIGVLDQR EYLTTLGQRLAHISTDPRLAKAIVL AAIFRCLHPLLVVVSCLTRDPFSSS LQNRAEVDKVKALLSHDSGSDHLAF VRAVAGWEEVLRWQDRSSRENYLEE NLLYAPSLRFIHGLIKQFSENIYEA FLVGKPSDCTLASAQCNEYSEEEEL VKGVLMAGLYPNLIQVRQGKVTRQG KFKPNSVTYRTKSGNILLHKSTINR EATRLRSRWLTYFMAVKSNGSVFVR DSSQVHPLAVLLLTDGDVHIRDDGR RATISLSDSDLLRLEGDSRTVRLLK ELRRALGRMVERSLRSELAALPPSV QEEHGQLLALLAELLRGPCGSFDVR KTADDTRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: DHX30 DExH-box helicase 30 [ Homo sapiens (human) ]
• Official Symbol: DHX30
• Synonyms: DHX30; DEAH (Asp-Glu-Ala-His) box polypeptide 30; DDX30, DEAD/H (Asp Glu Ala Asp/His) box polypeptide 30; putative ATP-dependent RNA helicase DHX30; FLJ11214; KIAA0890; DEAH box protein 30; retina co-repressor; ATP-dependent RNA helicase DHX30; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 30; DDX30; RETCOR
• Gene ID: 22907
• mRNA Refseq: NM_014966
• Protein Refseq: NP_055781
• MIM: 616423
• UniProt ID: Q7L2E3