Recombinant Human DEFB1 protein, His & GST-tagged

• Cat.No.: DEFB1-6876H
• Product Overview: Recombinant Human DEFB1 aa. (Gly22~Lys68 (Accession # P60022)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis.
• Source: E. coli
• Species: Human
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 37.5kDa.
• Protein length: Gly22~Lys68
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 7.6.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in ddH2O.

Gene Information

• Gene Name: DEFB1 defensin beta 1 [ Homo sapiens (human) ]
• Official Symbol: DEFB1
• Synonyms: BD1; HBD1; DEFB-1; DEFB101; BD-1; beta-defensin 1
• Gene ID: 1672
• mRNA Refseq: NM_005218.3
• Protein Refseq: NP_005209.1
• UniProt ID: P60022