Recombinant Human Decapping Enzyme, Scavenger, His-tagged

• Cat.No.: DCPS-1680H
• Product Overview: Recombinant human DCPS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Cat. No.: DCPS-1680H
• Description: DCPS, also known as scavenger mRNA-decapping enzyme, belongs to the HIT family. It is necessary for the complete degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. A scavenger decapping enzyme, DcpS was shown to hydrolyze the residual m7GpppN cap structure after the complete 3?–5? degradation of the mRNA by the exosome. DcpS releases m7GMP and is unable to cleave cap structures attached to a long RNA chain.
• Concentration: 0.5 mg/ml
• Source: Human
• Host: E.coli
• Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, with 20% glycerol and 1mM DTT.
• Purity: > 90% by SDS - PAGE
• Sequence: 1-337 amino acids: MGSSHHHHHH SSGLVPRGSH MADAAPQLGK RKRELDVEEA HAASTEEKEA GVGNGTCAPV RLPFSGFRLQ KVLRESARDK IIFLHGKVNE ASGDGDGEDA VVILEKTPFQ VEQVAQLLTG SPELQLQFSN DIYSTYHLFP PRQLNDVKTT VVYPATEKHL QKYLRQDLRL IRETGDDYRN ITLPHLESQS LSIQWVYNIL DKKAEADRIV FENPD
• Molecular Mass: 40.8 kDa
• Applications: SDS PAGE
• Storage: Store at 4 deg C for short term storage. Aliquot and store at -20 deg C or -70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: DCPS decapping enzyme, scavenger [ Homo sapiens ]
• Official Symbol: DCPS
• Synonyms: DCPS; HSL1; HINT-5; HSPC015; decapping enzyme, scavenger; scavenger mRNA-decapping enzyme DcpS; DCS-1; OTTHUMP00000231113; mRNA decapping enzyme; heat shock-like protein 1; histidine triad protein member 5; hint-related 7meGMP-directed hydrolase; homolog of C. elegans 7meGMP-directed hydrolase dcs-1; EC 3.
• Gene ID: 28960
• mRNA Refseq: NM_014026
• Protein Refseq: NP_054745
• MIM: 610534
• UniProt ID: Q96C86
• Chromosome Location: 11q24.2
• Pathway: Deadenylation-dependent mRNA decay; Metabolism of RNA; Metabolism of Mrna; RNA degradation; mRNA Decay by 3" to 5" Exoribonuclease
• Function: hydrolase activity; protein binding