Recombinant Human DARS2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: DARS2-2020H
• Product Overview: DARS2 MS Standard C13 and N15-labeled recombinant protein (NP_060592) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL).
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 73.4 kDa
• AA Sequence: MYFPSWLSQLYRGLSRPIRRTTQPIWGSLYRSLLQSSQRRIPEFSSFVVRTNTCGELRSSHLGQEVTLCGWIQYRRQNTFLVLRDFDGLVQVIIPQDESAASVKKILCEAPVESVVQVSGTVISRPAGQENPKMPTGEIEIKVKTAELLNACKKLPFEIKNFVKKTEALRLQYRYLDLRSFQMQYNLRLRSQMVMKMREYLCNLHGFVDIETPTLFKRTPGGAKEFLVPSREPGKFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPEFTQIDIEMSFVDQTGIQSLIEGLLQYSWPNDKDPVVVPFPTMTFAEVLATYGTDKPDTRFGMKIIDISDVFRNTEIGFLQDALSKPHGTVKAICIPEGAKYLKRKDIESIRNFAADHFNQEILPVFLNANRNWNSPVANFIMESQRLELIRLMETQEEDVVLLTAGEHNKACSLLGKLRLECADLLETRGVVLRDPTLFSFLWVVDFPLFLPKEENPRELESAHHPFTAPHPSDIHLLYTEPKKARSQHYDLVLNGNEIGGGSIRIHNAELQRYILATLLKEDVKMLSHLLQALDYGAPPHGGIALGLDRLICLVTGSPSIRDVIAFPKSFRGHDLMSNTPDSVPPEELKPYHIRVSKPTDSKAERAHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: DARS2 aspartyl-tRNA synthetase 2, mitochondrial [ Homo sapiens (human) ]
• Official Symbol: DARS2
• Synonyms: DARS2; aspartyl-tRNA synthetase 2, mitochondrial; aspartate--tRNA ligase, mitochondrial; aspartate tRNA ligase 2; mitochondrial; FLJ10514; aspartate tRNA ligase 2, mitochondrial; aspartyl-tRNA synthetase, mitochondrial; LBSL; ASPRS; MT-ASPRS; RP3-383J4.2
• Gene ID: 55157
• mRNA Refseq: NM_018122
• Protein Refseq: NP_060592
• MIM: 610956
• UniProt ID: Q6PI48