Recombinant Human D2HGDH protein, His & T7-tagged

• Cat.No.: D2HGDH-1657H
• Product Overview: Recombinant Human D2HGDH aa. (Arg56~Asp232 (Accession # Q8N465)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes D-2hydroxyglutarate dehydrogenase, a mitochondrial enzyme belonging to the FAD-binding oxidoreductase/transferase type 4 family. This enzyme, which is most active in liver and kidney but also active in heart and brain, converts D-2-hydroxyglutarate to 2-ketoglutarate. Mutations in this gene are present in D-2-hydroxyglutaric aciduria, a rare recessive neurometabolic disorder causing developmental delay, epilepsy, hypotonia, and dysmorphic features.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 22.8kDa
• Protein length: Arg56~Asp232
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 6.7

Gene Information

• Gene Name: D2HGDH D-2-hydroxyglutarate dehydrogenase [ Homo sapiens (human) ]
• Official Symbol: D2HGDH
• Synonyms: D2HGDH; D-2-hydroxyglutarate dehydrogenase; D2HGD; D-2-hydroxyglutarate dehydrogenase, mitochondrial
• Gene ID: 728294
• mRNA Refseq: NM_001287249.1
• Protein Refseq: NP_001274178.1
• UniProt ID: Q8N465