Recombinant Human Cystathionase (cystathionine gamma-lyase), His-tagged

• Cat.No.: CTH-1333H
• Product Overview: Recombinant human CTH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: CTH (Cystathionine gamma-lyase or cystathionase) belongs to the trans-sulfuration enzymes family. This protein is an enzyme which breaks down cystathionine into cysteine and alpha-ketobutyrate. CTH catalyzes the last step in the transsulfuration pathway from methionine to cysteine.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 2 mM DTT, 10% glycerol, 100 mM NaCl.
• Molecular Weight: 46.7 kDa (425 aa), confirmed by MALDI-TOF
• Purity: > 95 % by SDS-PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MQEKDASSQG FLPHFQHFAT QAIHVGQDPE QWTSRAVVPP ISLSTTFKQG APGQHSGFEY SRSGNPTRNC LEKAVAALDG AKYCLAFASG LAATVTITHL LKAGDQIICM DDVYGGTNRY FRQVASEFGL KISFVDCSKI KLLEAAITPE TKLVWIETPT NPTQKVIDIE GCAHIVHKHG DIILVVDNTF MSPYFQRPLA LGADISMYSA TKYMNGHSDV VMGLVSVNCE SLHNRLRFLQ NSLGAVPSPI DCYLCNRGLK TLHVRMEKHF KNGMAVAQFL ESNPWVEKVI YPGLPSHPQH ELVKRQCTGC TGMVTFYIKG TLQHAEIFLK NLKLFTLAES LGGFESLAEL PAIMTHASVL KNDRDVLGIS DTLIRLSVGL EDEEDLLEDL DQALKAAHPP SGSHS
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Cysteine and methionine metabolism; Glycine; L-cysteine degradation II; Metabolic pathways; Nitrogen metabolism; Selenium Pathway; Selenocompound metabolism; cysteine biosynthesis II; glutathione-mediated detoxification; homocysteine degradation I

Gene Information

• Gene Name: CTH cystathionase (cystathionine gamma-lyase) [ Homo sapiens ]
• Official Symbol: CTH
• Synonyms: CTH; cystathionase (cystathionine gamma-lyase); MGC9471; cystathionine gamma-lyase; OTTHUMP00000010945; OTTHUMP00000010946; gamma-cystathionase; homoserine deaminase; cysteine desulfhydrase; homoserine dehydratase; EC=4.4.1.1
• Gene ID: 1491
• mRNA Refseq: NM_001190463
• Protein Refseq: NP_001177392
• MIM: 607657
• UniProt ID: P32929
• Chromosome Location: 1p31.1
• Function: L-cysteine desulfhydrase activity; cystathionine beta-lyase activity; cystathionine gamma-lyase activity; lyase activity; pyridoxal phosphate binding