Recombinant Human CYP4F12 293 Cell Lysate

• Cat.No.: CYP4F12-7102HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for cytochrome P450, family 4, subfamily F, polypeptide 12 (CYP4F12) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: CYP4F12 cytochrome P450, family 4, subfamily F, polypeptide 12 [ Homo sapiens ]
• Official Symbol: CYP4F12
• Synonyms: CYP4F12; cytochrome P450, family 4, subfamily F, polypeptide 12; cytochrome P450, subfamily IVF, polypeptide 12; cytochrome P450 4F12; CYPIVF12; F22329_1
• Gene ID: 66002
• mRNA Refseq: NM_023944
• Protein Refseq: NP_076433
• MIM: 611485
• UniProt ID: Q9HCS2
• Chromosome Location: 19p13.1
• Pathway: Biological oxidations, organism-specific biosystem; Cytochrome P450 - arranged by substrate type, organism-specific biosystem; Fatty acids, organism-specific biosystem; Metabolism, organism-specific biosystem; Phase 1 - Functionalization of compounds, organism-specific biosystem; Tryptophan metabolism, organism-specific biosystem
• Function: alkane 1-monooxygenase activity; arachidonic acid epoxygenase activity; aromatase activity; electron carrier activity; heme binding; leukotriene-B4 20-monooxygenase activity; metal ion binding; monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; vitamin-K-epoxide reductase (warfarin-sensitive) activity