Recombinant Human CYP21A2 293 Cell Lysate

• Cat.No.: CYP21A2-7123HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for cytochrome P450, family 21, subfamily A, polypeptide 2 (CYP21A2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: CYP21A2 cytochrome P450, family 21, subfamily A, polypeptide 2 [ Homo sapiens ]
• Official Symbol: CYP21A2
• Synonyms: CYP21A2; cytochrome P450, family 21, subfamily A, polypeptide 2; CYP21, CYP21B, cytochrome P450, subfamily XXIA (steroid 21 hydroxylase, congenital adrenal hyperplasia), polypeptide 2; steroid 21-hydroxylase; CA21H; CAH1; CPS1; P450c21B; Steroid 21 monooxygenase; 21-OHase; cytochrome P450 21; cytochrome P450 XXI; cytochrome P450-C21B; steroid 21-monooxygenase; cytochrome P450, subfamily XXIA (steroid 21-hydroxylase, congenital adrenal hyperplasia), polypeptide 2; CYP21; CYP21B; MGC150536; MGC150537
• Gene ID: 1589
• mRNA Refseq: NM_000500
• Protein Refseq: NP_000491
• MIM: 613815
• UniProt ID: P08686
• Chromosome Location: 6p21.3
• Pathway: Biological oxidations, organism-specific biosystem; C21-Steroid hormone biosynthesis, progesterone =>corticosterone/aldosterone, organism-specific biosystem; C21-Steroid hormone biosynthesis, progesterone => corticosterone/aldosterone, conserved biosystem; C21-Steroid hormone biosynthesis, progesterone => cortisol/cortisone, organism-specific biosystem
• Function: electron carrier activity; heme binding; metal ion binding; monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; steroid 21-monooxygenase activity; steroid binding; steroid hydroxylase activity