Recombinant Human CD28 Protein (Asn19-Pro152), N-His-SUMO tagged, Animal-free, Carrier-free

• Cat.No.: CD28-322H
• Product Overview: Recombinant Human CD28 Protein (Asn19-Pro152) with N-His-SUMO tag was expressed in HEK293 and Animal-free as well as Carrier-free.

Specification

• Description: CD28, a well-known costimulatory receptor expressed on T cells, is a type I transmembrane glycoprotein of the Ig superfamily. When the T cells receive the first signal delivered by APCs (antigen-presenting cells), it initiates signaling cascades, which favor T cell survival and proliferation. It has been demonstrated that patients with lung cancer who responded to PD-1 therapy had more CD28+ T cells, suggesting that CD28 may predict treatment response.
• Source: HEK293 cell
• Species: Human
• Tag: N-His-SUMO
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 28.61 kDa.
  The protein migrates as 48-62 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Asn19-Pro152
• Endotoxin: < 1 EU/μg of the protein by the LAL method.
• Purity: >95% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: CD28 CD28 molecule [ Homo sapiens (human) ]
• Official Symbol: CD28
• Synonyms: CD28; CD28 molecule; CD28 antigen (Tp44); T-cell-specific surface glycoprotein CD28; T cell specific surface glycoprotein; CD28 antigen; Tp44; MGC138290
• Gene ID: 940
• mRNA Refseq: NM_001243077
• Protein Refseq: NP_001230006
• MIM: 186760
• UniProt ID: P10747