Recombinant Human CYBB, His-tagged

• Cat.No.: CYBB-11752H
• Product Overview: Recombinant Human CYBB protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 422-577a.a.
• Description: Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cells respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.

Gene Information

• Gene Name: CYBB cytochrome b-245, beta polypeptide [ Homo sapiens ]
• Official Symbol: CYBB
• Synonyms: CYBB; cytochrome b-245, beta polypeptide; CGD, chronic granulomatous disease; cytochrome b-245 heavy chain; GP91 PHOX; NOX2; CGD91-phox; NADPH oxidase 2; p22 phagocyte B-cytochrome; cytochrome b558 subunit beta; cytochrome b(558) subunit beta; neutrophil cytochrome b 91 kDa polypeptide; heme-binding membrane glycoprotein gp91phox; superoxide-generating NADPH oxidase heavy chain subunit; CGD; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
• Gene ID: 1536
• mRNA Refseq: NM_000397
• Protein Refseq: NP_000388
• MIM: 300481
• UniProt ID: P04839
• Chromosome Location: Xp21.1
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing-Cross presentation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Cross-presentation of particulate exogenous antigens (phagosomes), organism-specific biosystem; Immune System, organism-specific biosystem; Leukocyte transendothelial migration, organism-specific biosystem
• Function: contributes_to electron carrier activity; flavin adenine dinucleotide binding; heme binding; metal ion binding; oxidoreductase activity; protein binding; protein heterodimerization activity; contributes_to superoxide-generating NADPH oxidase activity; sup