Recombinant Human CTD (Carboxy-Terminal Domain, RNA Polymerase II, Polypeptide A) Small, Phosphatase 1, His-tagged

• Cat.No.: CTDSP1-975H
• Product Overview: Recombinant human CTDSP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Carboxy-terminal domain, RNA polymerase II, polypeptide A phosphatase 1, also known as CTDSP1, is a class 2C phosphatase with activity dependent on the conserved DxD motif. CTDSP1 preferentially catalyzes the dephosphorylation of "Ser-5" within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Also it negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation.
• Formulation: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl
• Purity: > 90% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Sequences of animo acids: MGSSHHHHHH SSGLVPRGSHMDSSAVITQI SKEEARGPLR GKGDQKSAAS QKPRSRGILH SLFCCVCRDD GEALPAHSGA PLLVEENGAIPKTPVQYLLP EAKAQDSDKI CVVIDLDETL VHSSFKPVNN ADFIIPVEID GVVHQVYVLK RPHVDEFLQR MGELFECVLF TASLAKYADP VADLLDKWGA FRARLFRESC VFHRGNYVKD LSRLGRDLRR VLILDNSPAS YVFHPDNAVP VASWFDNMSD TELHDLLPFF EQLSRVDDVYSVLRQPRPGS
• Storgage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: CTDSP1 CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 1 [ Homo sapiens ]
• Synonyms: CTDSP1;CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small,phosphatase 1;small CTD phosphatase 1;NLI-interacting factor 3;small C-terminal domain phosphatase 1;nuclear LIM interactor-interacting factor 3;carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1;SCP1; NLIIF; CTDSP1;nuclear LIMinteractor-interacting factor;small CTD phosphatase 1;EC 3.1.3.16;NIF3;NLI-IF;CTD (carboxy-terminal domain, RNA polymerase II,NLI-interacting factor 3
• Gene ID: 58190
• mRNA Refseq: NM_021198
• Protein Refseq: NP_067021
• MIM: 605323
• UniProt ID: Q9GZU7
• Chromosome Location: 2q35
• Function: CTD phosphatase activity;hydrolase activity;metal ion binding;phosphoprotein phosphatase activity;protein binding