Recombinant Human CSF2 protein, His-tagged

• Cat.No.: CSF2-453H
• Product Overview: Recombinant Human CSF2 (P04141) aa. (Ala18~Glu144) fused with His tag was produced in HEK293F cells.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Ala18~Glu144
• Description: The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 16.1kDa; Accurate Molecular Mass: 19-26kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 95%
• Characteristic: Isoelectric point is 5.2.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200ug/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: CSF2 colony stimulating factor 2 [ Homo sapiens (human) ]
• Official Symbol: CSF2
• Synonyms: CSF2; colony stimulating factor 2; GMCSF; granulocyte-macrophage colony-stimulating factor; CSF; colony stimulating factor 2 (granulocyte-macrophage); granulocyte macrophage-colony stimulating factor; molgramostin; sargramostim
• Gene ID: 1437
• mRNA Refseq: NM_000758.3
• Protein Refseq: NP_000749.2
• UniProt ID: P04141