Recombinant Human CRKL, His-tagged

• Cat.No.: CRKL-6997H
• Product Overview: Recombinant full length human CRKL was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Specification

• Description: CRKL or v-crk sarcoma virus CT10 oncogene homolog (avian)-like is a protein kinase containing SH2 and SH3 (src homology) domains that activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase which plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic. CRKL is also required for normal cellular responses to Fgf8, including survival and migration, Erk activation, and target gene expression.
• Source: Sf9 Cells
• Species: Human
• Tag: His
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Molecular Mass: ~39kDa
• Protein length: Full length
• Purity: >80%
• Applications: Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.2μg/μl

Gene Information

• Gene Name: CRKL v-crk sarcoma virus CT10 oncogene homolog (avian)-like [ Homo sapiens ]
• Official Symbol: CRKL
• Synonyms: CRKL; v-crk sarcoma virus CT10 oncogene homolog (avian)-like; v crk avian sarcoma virus CT10 oncogene homolog like; crk-like protein
• Gene ID: 1399
• mRNA Refseq: NM_005207
• Protein Refseq: NP_005198
• MIM: 602007
• UniProt ID: P46109
• Chromosome Location: 22q11.21
• Pathway: B Cell Receptor Signaling Pathway, organism-specific biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Chronic myeloid leukemia, organism-specific biosystem; Chronic myeloid leukemia, conserved biosystem
• Function: SH3/SH2 adaptor activity; protein binding; signal transducer activity