Recombinant Human CPB1 protein(Met1-Tyr417), His-tagged

• Cat.No.: CPB1-3175H
• Product Overview: Recombinant Human CPB1 (NP_001862.2) (Met 1-Tyr 417) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Protein length: 1-417 a.a.
• Form: Lyophilized from sterile 25mM MES, 0.1 M NaCl, pH 6.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg, as measured using the wavelength at 254 nm. The specific activity is >1000 pmoles/min/μg.
• Molecular Mass: The recombinant human CPB1 consists of 413 amino acids and has a predicted molecular mass of 47 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 45 kDa band.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CPB1 carboxypeptidase B1 (tissue) [ Homo sapiens ]
• Official Symbol: CPB1
• Synonyms: CPB1; carboxypeptidase B1 (tissue); carboxypeptidase B; pancreatic carboxypeptidase B; protaminase; tissue carboxypeptidase B; procarboxypeptidase B; pancreas-specific protein; CPB; PASP; PCPB; DKFZp779K1333
• Gene ID: 1360
• mRNA Refseq: NM_001871
• Protein Refseq: NP_001862
• MIM: 114852
• UniProt ID: P15086