Recombinant Human CPB1, His-tagged

• Cat.No.: CPB1-7752H
• Product Overview: Recombinant Human Carboxypeptidase B/CPB1 is produced with our mammalian cells expression system in HEK293. The target protein is expressed with sequence (His16Tyr417) of Human CPB1 fused with a polyhistidine tag at the Cterminus.

Specification

• Description: Carboxypeptidase B1 is an exopeptidase which specifically cleaves the Cterminal Arg and Lys residues with a preference for Arg. Expressed mainly in pancreas, CPB1 is a useful serum marker for acute pancreatitis and pancreatic graft rejection.Human CPB1 consists of a signal peptide, a pro region, and a mature chain. The purified rhCPB1 corresponds to the pro form, which can be activated by trypsin, the only pancreatic protease capable of generating active enzyme from the zymogen in vitro.
• Source: HEK293 Cells
• Species: Human
• Tag: His
• Form: Supplied as a 0.2 μM filtered solution of 20mM MES, 150mM NaCl, pH 5.5
• AA Sequence: HHGGEHFEGE KVFRVNVEDE NHINIIRELA STTQIDFWKP DSVTQIKPHS TVDFRVKAED TVTVENVLK QNELQYKVLI SNLRNVVEAQ FDSRVRATGH SYEKYNKWET IEAWTQQVAT E NPALISRS VIGTTFEGRA IYLLKVGKAG QNKPAIFMDC GFHAREWISP AFCQWFVREA VR TYGREIQ VTELLDKLDF YVLPVLNIDG YIYTWTKSRF WRKTRSTHTG SSCIGTDPNR NFD AGWCEI GASRNPCDET
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Notes: For research use only.
• Storage: Store at less than 20°C. stable for 6 months after receipt.Please minimize freezethaw cycles.

Gene Information

• Gene Name: CPB1 carboxypeptidase B1 (tissue) [ Homo sapiens ]
• Official Symbol: CPB1
• Synonyms: CPB1; carboxypeptidase B1 (tissue); carboxypeptidase B; pancreatic carboxypeptidase B; protaminase; tissue carboxypeptidase B; procarboxypeptidase B; pancreas-specific protein; CPB; PASP; PCPB; DKFZp779K1333
• Gene ID: 1360
• mRNA Refseq: NM_001871
• Protein Refseq: NP_001862
• MIM: 114852
• UniProt ID: P15086
• Chromosome Location: 3q24
• Pathway: Pancreatic secretion, organism-specific biosystem; Pancreatic secretion, conserved biosystem; Protein digestion and absorption, organism-specific biosystem; Protein digestion and absorption, conserved biosystem
• Function: carboxypeptidase activity; metal ion binding; metallocarboxypeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding