Recombinant Human COL4A3 Protein, His/GST-tagged

• Cat.No.: COL4A3-334H
• Product Overview: Recombinant Human COL4A3(Leu1428~His1670) fused with His/GST tag at N-terminal was expressed in E. coli.

Specification

• Description: Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.
• Source: E. coli
• Species: Human
• Tag: His&GST
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Molecular Mass: 56.79kDa
• Protein length: Leu1428~His1670
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others

Gene Information

• Gene Name: COL4A3 collagen, type IV, alpha 3 (Goodpasture antigen) [ Homo sapiens ]
• Official Symbol: COL4A3
• Synonyms: COL4A3; collagen, type IV, alpha 3 (Goodpasture antigen); collagen alpha-3(IV) chain; tumstatin; collagen IV, alpha-3 polypeptide
• Gene ID: 1285
• mRNA Refseq: NM_000091
• Protein Refseq: NP_000082
• MIM: 120070
• UniProt ID: Q01955