Recombinant Human COL18A1 Protein, His-tagged

• Cat.No.: COL18A1-1274H
• Product Overview: Recombinant Human COL18A1 Protein (His1154-Ser1335) with N-His tag.

Specification

• Description: This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants.
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 21.58 kDa
  Accurate Molecular Mass: 21 kDa
• Protein length: His1154-Ser1335
• Purity: > 90%
• Applications: Cell culture; Activity Assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: COL18A1 collagen type XVIII alpha 1 chain [ Homo sapiens (human) ]
• Official Symbol: COL18A1
• Synonyms: COL18A1; collagen type XVIII alpha 1 chain; KS; KNO; GLCC; KNO1; collagen alpha-1(XVIII) chain; antiangiogenic agent; collagen, type XVIII, alpha 1; endostatin; multi-functional protein MFP
• Gene ID: 80781
• mRNA Refseq: NM_030582
• Protein Refseq: NP_085059
• MIM: 120328
• UniProt ID: P39060