Recombinant Human COL17A1 293 Cell Lysate

• Cat.No.: COL17A1-7378HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for collagen, type XVII, alpha 1 (COL17A1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of 100ug gene-specific cell lysate in RIPA buffer, 1 vial of 100ug whole HEK293T cell lysate in RIPA buffer, and 1 vial of 0.25 ml 2X SDS sample buffer (125mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 100mM DTT).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: COL17A1 collagen, type XVII, alpha 1 [ Homo sapiens ]
• Official Symbol: COL17A1
• Synonyms: COL17A1; collagen, type XVII, alpha 1; BPAG2; collagen alpha-1(XVII) chain; BP180; alpha 1 type XVII collagen; collagen XVII, alpha-1 polypeptide; 180 kDa bullous pemphigoid antigen 2; bullous pemphigoid antigen 2 (180kD); bA16H23.2 (collagen, type XVII, alpha 1 (BP180)); BPA-2; LAD-1; BA16H23.2; FLJ60881; KIAA0204
• Gene ID: 1308
• mRNA Refseq: NM_000494
• Protein Refseq: NP_000485
• MIM: 113811
• UniProt ID: Q9UMD9
• Chromosome Location: 10q24.3
• Pathway: Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Cell junction organization, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Protein digestion and absorption, organism-specific biosystem; Protein digestion and absorption, conserved biosystem; Type I hemidesmosome assembly, organism-specific biosystem; a6b1 and a6b4 Integrin signaling, organism-specific biosystem
• Function: protein binding