Recombinant Human COCH protein(Glu25-Gln550), His-tagged

• Cat.No.: COCH-3207H
• Product Overview: Recombinant Human COCH (NP_001128530.1) (Glu 25-Gln 550) was expressed in HEK293, with a polyhistidine tag at the N-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 25-550 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human COCH consists of 542 amino acids and has a calculated molecular mass of 59.4 kDa. In SDS-PAGE under reducing conditions, rhCOCH migrates as three bands with apparent molecular mass of 66, 48 and 18 kDa, corresponding to the alternative splicing isoforms.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: COCH coagulation factor C homolog, cochlin (Limulus polyphemus) [ Homo sapiens ]
• Official Symbol: COCH
• Synonyms: COCH; coagulation factor C homolog, cochlin (Limulus polyphemus); coagulation factor C (Limulus polyphemus homolog); cochlin , DFNA9, DFNA31; cochlin; COCH 5B2; DFNA9; COCH5B2; COCH-5B2
• Gene ID: 1690
• mRNA Refseq: NM_001135058
• Protein Refseq: NP_001128530
• MIM: 603196
• UniProt ID: O43405

Case Study

Case 1: Ikezono T, et al. Biochim Biophys Acta. 2001

Researchers aimed to understand hereditary hearing loss by analyzing inner ear proteins using two-dimensional gel electrophoresis. They found interesting features in a protein related to the COCH gene, linked to hearing issues like DFNA9 and potentially Ménière's disease. This Coch protein constitutes a significant portion of inner ear proteins and shows considerable diversity, suggesting complex processing in the body. While much is known about the genes causing hearing loss, there's less information about the proteins they produce. This research highlights the Coch protein, offering insights into hearing and balance disorders.

Fig1. Enlarged image of the boxed area of the 2D gel in panel a.

Fig2. Peptide maps by in-gel digestion with Lys-C of p63-1 to 6, p44-1 to 5 and p40-1 to 3.

Case 2: Picciani R, et al. Prog Retin Eye Res. 2007

Aqueous humor, produced in the eye's ciliary epithelium, is key for eye function. Normally, its flow is well-balanced, but diseases can disrupt this. The trabecular meshwork (TM) in the anterior chamber, which acts like a filter, offers most flow resistance. Changes in the TM affect this filtering, though how it works isn't fully known. Cochlin, a protein, shows up in glaucomatous but not in healthy TMs, confirmed by various analyses. Cochlin forms multimers due to shear stress and environmental changes. It's found with mucopolysaccharides in glaucomas and in ears with DNFA9, a hearing issue linked to fluid changes. Cochlin seems to influence TM cell clumping, hinting at roles in adhesion and perhaps mechanosensation.

Fig1. Coommassie Blue-stained SDS-PAGE of HA-tagged recombinant cochlin and Notch.

Fig2. Immunoprecipitation (IP) was carried out using three different methods.

Application

Recombinant COCH protein is making waves in the study of ear health and hearing solutions. This protein is crucial for the inner ear's structure and function. By exploring COCH, we might make progress in treating hearing issues like Meniere's disease or hearing loss that comes with getting older. It's like giving researchers a fresh map to better tackle hearing problems, offering hope to those who struggle with these challenges. Beyond just ears, the COCH protein might play a role in other areas of health. Since it helps with how cells communicate and maintain structure, there could be new ways to treat conditions where these functions go awry. Studying COCH's role in the body might lead to innovative solutions for keeping cells healthy, making it an exciting target for developing new medical therapies.

Fig1. Cochlin is the dual-armed immune-regulator of the inner ear. (Hyeong-Jun Rhyu, 2020)

Fig2. Schematic Model Illustrating that Cleaved Cochlin (LCCL) Plays a Dual Role to Trap Bacterial Pathogens and Recruit Immune Cells to Improve the Inner Ear Function. (Kensei Komatsu, 2019)