Recombinant Human COCH protein(Glu25-Gln550), His-tagged

• Cat.No.: COCH-3207H
• Product Overview: Recombinant Human COCH (NP_001128530.1) (Glu 25-Gln 550) was expressed in HEK293, with a polyhistidine tag at the N-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Protein length: 25-550 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human COCH consists of 542 amino acids and has a calculated molecular mass of 59.4 kDa. In SDS-PAGE under reducing conditions, rhCOCH migrates as three bands with apparent molecular mass of 66, 48 and 18 kDa, corresponding to the alternative splicing isoforms.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: COCH coagulation factor C homolog, cochlin (Limulus polyphemus) [ Homo sapiens ]
• Official Symbol: COCH
• Synonyms: COCH; coagulation factor C homolog, cochlin (Limulus polyphemus); coagulation factor C (Limulus polyphemus homolog); cochlin , DFNA9, DFNA31; cochlin; COCH 5B2; DFNA9; COCH5B2; COCH-5B2
• Gene ID: 1690
• mRNA Refseq: NM_001135058
• Protein Refseq: NP_001128530
• MIM: 603196
• UniProt ID: O43405