Recombinant Human CLOCK protein, His & T7-tagged

• Cat.No.: CLOCK-1526H
• Product Overview: Recombinant Human CLOCK aa. (Ala34~Ala379 (Accession # O15516)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 44.0kDa
• Protein length: Ala34~Ala379
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 6.2

Gene Information

• Gene Name: CLOCK clock circadian regulator [ Homo sapiens (human) ]
• Official Symbol: CLOCK
• Synonyms: CLOCK; clock circadian regulator; KAT13D; bHLHe8; circadian locomoter output cycles protein kaput; circadian locomoter output cycles kaput protein; class E basic helix-loop-helix protein 8; clock homolog
• Gene ID: 9575
• mRNA Refseq: NM_001267843.1
• Protein Refseq: NP_001254772.1
• UniProt ID: O15516