Recombinant Human cholinergic receptor, nicotinic, alpha 6 (neuronal), His-tagged

• Cat.No.: CHRNA6-475H
• Product Overview: CHRNA6, 26-239aa Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 26-239 a.a.
• Description: Cholinergic receptor, nicotinic, alpha 6, also known as CHRNA6, belongs to the nicotinic acetylcholine receptors (nAChRs) family. nAChRs are heteropentameric ligand-gated ion channels resulting from the assembly of alpha and beta protein subunits. This ion channel is gated by the binding of acetylcholine to the assembled receptor. Alpha 6 is often found associated with beta 3, beta 2, and alpha 4 subunits. CHRNA6 receptors have a relatively selective localization to the nigrostriatal pathway where they function to mediate dopamine release. Recombinant human CHRNA6 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol
• Molecular Mass: 29.3 kDa (250aa)
• AA Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSKGCV GCATEERLFH KLFSHYNQFI RPVENVSDPV TVHFEVAITQ LANVDEVNQI METNLWLRHI WNDYKLRWDP MEYDGIETLR VPADKIWKPD IVLYNNAVGD FQVEGKTKAL LKYNGMITWT PPAIFKSSCP MDITFFPFDH QNCSLKFGSW TYDKAEIDLL IIGSKVDMND FWENSEWEII DASGYKHDIK YNCCEEIYTD ITYSFYIRRL
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: CHRNA6 cholinergic receptor, nicotinic, alpha 6 (neuronal) [ Homo sapiens ]
• Official Symbol: CHRNA6
• Synonyms: CHRNA6; cholinergic receptor, nicotinic, alpha 6 (neuronal); cholinergic receptor, nicotinic, alpha polypeptide 6; neuronal acetylcholine receptor subunit alpha-6; acetylcholine receptor; nicotinic; alpha 6 (neuronal); acetylcholine receptor, nicotinic, alpha 6 (neuronal); CHNRA6
• Gene ID: 8973
• mRNA Refseq: NM_001199279
• Protein Refseq: NP_001186208
• MIM: 606888
• UniProt ID: Q15825
• Chromosome Location: 8p22
• Pathway: Acetylcholine Binding And Downstream Events, organism-specific biosystem; Activation of Nicotinic Acetylcholine Receptors, organism-specific biosystem; Cholinergic synapse, organism-specific biosystem; Highly calcium permeable nicotinic acetylcholine receptors, organism-specific biosystem; Highly calcium permeable postsynaptic nicotinic acetylcholine receptors, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem; Neuroactive ligand-receptor interaction, conserved biosystem
• Function: acetylcholine receptor activity; acetylcholine-activated cation-selective channel activity; extracellular ligand-gated ion channel activity; ion channel activity; receptor activity