Recombinant Human CHI3L1 protein(Tyr22-Thr383), His-tagged

• Cat.No.: CHI3L1-657H
• Product Overview: Recombinant Human CHI3L1 protein(P36222)(Tyr22-Thr383) is expressed from HEK293 with His tag at the C-Terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Protein length: Tyr22-Thr383
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human YKL-40, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-YKL-40 Antibody, hFc Tag with the EC50 of 17.8ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 42-50 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  -80°C for 3-6 months after reconstitution.
  2-8°C for 2-7 days after reconstitution.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CHI3L1 chitinase 3-like 1 (cartilage glycoprotein-39) [ Homo sapiens ]
• Official Symbol: CHI3L1
• Synonyms: CHI3L1; chitinase 3-like 1 (cartilage glycoprotein-39); chitinase-3-like protein 1; GP39; YKL40; 39 kDa synovial protein; cartilage glycoprotein 39; ASRT7; GP-39; CGP-39; YKL-40; YYL-40; HC-gp39; HCGP-3P; hCGP-39; FLJ38139; DKFZp686N19119
• Gene ID: 1116
• mRNA Refseq: NM_001276
• Protein Refseq: NP_001267
• MIM: 601525
• UniProt ID: P36222