Recombinant Human Charged Multivesicular Body Protein 5, His-tagged

• Cat.No.: CHMP5-7172H
• Product Overview: Recombinant human CHMP5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 27 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-219aa
• Description: CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol.
• Molecular Mass: 27 kDa (243aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMNRLFG KAKPKAPPPS LTDCIGTVDS RAESIDKKIS RLDAELVKYK DQIKKMREGP AKNMVKQKAL RVLKQKRMYE QQRDNLAQQS FNMEQANYTI QSLKDTKTTV DAMKLGVKEM KKAYKQVKID QIEDLQDQLE DMMEDANEIQ EALSRSYGTP ELDEDDLEAE LDALGDELLA DEDSSYLDEA ASAPAIPEGV PTDTKNKDGV LVDEFGLPQI PAS
• Purity: >80% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: CHMP5 charged multivesicular body protein 5 [ Homo sapiens ]
• Official Symbol: CHMP5
• Synonyms: CHMP5; charged multivesicular body protein 5; C9orf83, chromatin modifying protein 5 , chromosome 9 open reading frame 83 , SNF7DC2; CGI 34; HSPC177; Vps60; hVps60; SNF7 domain containing 2; chromatin modifying protein 5; chromatin-modifying protein 5; SNF7 domain-containing protein 2; apoptosis-related protein PNAS-2; vacuolar protein sorting-associated protein 60; CGI-34; PNAS-2; C9orf83; SNF7DC2
• Gene ID: 51510
• mRNA Refseq: NM_001195536
• Protein Refseq: NP_001182465
• MIM: 610900
• UniProt ID: Q9NZZ3
• Chromosome Location: 9p13.3
• Pathway: ESCRT-III complex, organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; Endosomal Sorting Complex Required For Transport (ESCRT), organism-specific biosystem; Membrane Trafficking, organism-specific biosystem
• Function: protein binding