Recombinant Human CES1, His-tagged

• Cat.No.: CES1-901H
• Product Overview: Recombinant Human CES1(His19-Glu563) fussed with 10-his tag at C-terminal was expressed in Mouse NSO Cells.

Specification

• Species: Human
• Source: NS0
• Tag: His
• Description: Carboxylesterase 1 is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds. They have broadsubstrate specificity ranging from small molecule esters such as phenylester to long chain fatty acid esters and thioesters. They play a major role as determinants of pharmacokinetic behavior for most therapeutic agents containing an ester. By deesterification, they can activate or inactivate the agents. They also participate indetoxification of drugs such as cocaine and heroin in serum and liver. The resulting deesterified metabolites are secreted out in urine. They can also detoxifyorganophosphate and carbamate analogues used in agrochemicals or chemical nerve agents, such as malathion, sarin, tabun, and VX. In addition to the hydrolyticactivity, they can perform transesterification, a reaction important for cholesterol homeostasis. Carboxylesterase deficiency may be associated with nonHodgkinlymphoma or Bcell lymphocytic leukemia. CES1 shares the serine hydrolase fold observed in other esterases. CES1 possesses an endoplasmic reticulumretention signal (HIEL) at its Cterminus. The purified rhCES 1 lacks this signal, resulting in its secretion.
• Predicted Molecular Mass: 61 kDa
• N-terminal Sequence: His19
• Formulation: Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl.
• Purity: >95%, by SDSPAGE under reducing conditions and visualized by silver stain.
• SDS-PAGE: 61 kDa, reducing conditions
• Activity: Measured by its ability to hydrolyze pnitrophenylacetate. The specific activity is >6,000 pmol/min/μg, as measured under the described conditions.
• Endotoxin Level: <1.0 EU per 1 μg of the protein by the LAL method.
• Storage: Use a manual defrost freezer and avoid repeated freezethaw cycles. 6 months from date of receipt, 20 to 70°C as supplied. 3 months, 20 to 70°C under sterile conditions after opening.
• OfficialSymbol: CES1

Gene Information

• Gene Name: CES1 carboxylesterase 1 [ Homo sapiens ]
• Synonyms: CES1; carboxylesterase 1; CEH; REH; TGH; ACAT; CE-1; CES2; HMSE; SES1; HMSE1; PCE-1; hCE-1; liver carboxylesterase 1; egasyn; serine esterase 1; retinyl ester hydrolase; cocaine carboxylesterase; triacylglycerol hydrolase;carboxylesterase 2 (liver); brain carboxylesterase hBr1; cholesteryl ester hydrolase; monocyte/macrophage serine esterase; methylumbelliferyl-acetate deacetylase 1; acyl coenzyme A:cholesterol acyltransferase; acyl-coenzyme A:cholesterol acyltransferase; human monocyte/macrophage serine esterase 1; carboxylesterase 1 (monocyte/macrophage serine esterase 1); EC 3.1.1.1; EC 3.1.1.56; MT 1A; MT 2; MGC32848; MT2A
• Gene ID: 1066
• mRNA Refseq: NM_001266
• Protein Refseq: NP_001257
• MIM: 114835
• UniProt ID: P23141
• Chromosome Location: 16q22.2
• Pathway: Drug metabolism - other enzymes; E2F transcription factor network; Fluoropyrimidine Activity; Irinotecan Pathway; retinol biosynthesis
• Function: carboxylesterase activity; methyl indole-3-acetate esterase activity; methyl jasmonate esterase activity; methylumbelliferyl-acetate deacetylase activity; retinyl-palmitate esterase activity