Recombinant Human CELA1 protein, His & GST-tagged

• Cat.No.: CELA1-7029H
• Product Overview: Recombinant Human CELA1 aa. (Ser40~Asn258) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&GST
• ProteinLength: Ser40~Asn258
• Description: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, pancreatic elastase 1 is not expressed in the pancreas. To date, elastase 1 expression has only been detected in skin keratinocytes. Clinical literature that describes human elastase 1 activity in the pancreas or fecal material is actually referring to chymotrypsin-like elastase family, member 3B.
• Form: Freeze-dried powder
• Molecular Mass: 53kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 8.6.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200ug/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: CELA1 chymotrypsin like elastase family member 1 [ Homo sapiens (human) ]
• Official Symbol: CELA1
• Synonyms: CELA1; chymotrypsin like elastase family member 1; ELA1; chymotrypsin-like elastase family member 1; elastase 1, pancreatic; elastase-1; pancreatic elastase 1
• Gene ID: 1990
• mRNA Refseq: NM_001971.5
• Protein Refseq: NP_001962.3
• UniProt ID: Q9UNI1