Recombinant Human CDK8/CCNC/MED12 Protein (M1-Y464/M1-S283/M1-D100), GST/Flag/His-tagged

• Cat.No.: CDK8/CCNC/MED12-1633H
• Product Overview: Recombinant Human N-GST-3C-CDK8(1-464end)/pFastBac1-N-Flag-6His-TEV-Cyclin C(1-283end)/pFastBac1(kozak)-MED12(M1-D100)-TEV protein with a GST/Flag/His was expressed in Insect cell

Specification

• Species: Human
• Source: Insect Cells
• Tag: His&GST&Flag
• ProteinLength: M1-Y464/M1-S283/M1-D100
• Description: Cyclin-dependent kinase 8, Cell division protein kinase 8, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8, Protein kinase K35; Cyclin-C, SRB11 homolog, hSRB11; Mediator of RNA polymerase II transcription subunit 12, Activator-recruited cofactor 240 kDa component, ARC240, CAG repeat protein 45, Mediator complex subunit 12, OPA-containing protein, Thyroid hormone receptor-associated protein complex 230 kDa component, Trap230, Trinucleotide repeat-containing gene 11 protein Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation. Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.
• Form: Liquid
• Endotoxin: < 0.01 EU/μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM HEPES (pH 7.5), 150 mM NaCl, 5% glycerol.
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: CDK8 cyclin-dependent kinase 8 [ Homo sapiens (human) ]
• Official Symbol: CDK8
• Synonyms: Cyclin-dependent kinase 8; Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; Protein kinase K35; Cyclin-C; SRB11 homolog; hSRB11; Mediator of RNA polymerase II transcription subunit 12; Activator-recruited cofactor 240 kDa component; ARC240; CAG repeat protein 45; Mediator complex subunit 12; OPA-containing protein; Thyroid hormone receptor-associated protein complex 230 kDa component; Trap230; Trinucleotide repeat-containing gene 11 protein
• Gene ID: 1024
• mRNA Refseq: NM_001260
• Protein Refseq: NP_001251
• MIM: 603184
• UniProt ID: P49336