Active Recombinant Human General Transcription Factor IIA, 1, 19/37kDa

• Cat.No.: GTF2A1-1170H

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: The Transcription Factor IIA (TFIIA) has been shown to bind to the TBP-DNA complex and to increase the affinity of TBP for the TATA element. Human TFIIA consists of three subunits of 35 kDa (α-subunit), 19 kDa (β-subunit) and 12 kDa (γ-subunit). The α- and β-subunits are derived from the product, p55, of a single gene by an unknown mechanism. However, recombinant p55, in combination with a 12 kDa subunit (γ-subunit), retains native TFIIA activity. The p55 subunit of TFIIA is isolated from a strain of E. coli that contains the coding sequence of human p55 of TFIIA under the control of T7 promoter. The p12 subunit of TFIIA is isolated from a strain ofE. colithat contains the coding sequence for human TFIIA γ-subunit under the control of T7 promoter. Recombinant TFIIA can be used to super-shift TBPDNA complex in a gel mobility shift assay and to stimulate activator-dependent transcriptionin vitro. TFIIA is greater than 95% pure and does not contain any detectable protease, DNase, or RNase activities.
• Usage: For in vitro use only.
• Form: Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT, 20% glycerol.
• Activity: 1 ng is sufficient for a gel mobility shift assay in a 20 μl reaction, 20 ng are sufficient for in vitro transcription assay and 100 ng are sufficient for protein-protein interaction assays.
• Purity: > 95% by SDS-PAGE.
• Storage: Quality guaranteed for 12 months, store at -80℃. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: GTF2A1 general transcription factor IIA, 1, 19/37kDa [ Homo sapiens ]
• Synonyms: GTF2A1; general transcription factor IIA, 1, 19/37kDa; TF2A1; TFIIA; MGC129969; MGC129970; TFIIA alpha, p55, isoform 1; glucose regulated protein, 58kD pseudogene; TFIIA-42;Transcription initiation factor IIA subunit 1; Transcription initiation factor IIA alpha chain; TFIIA p35 subunit; TFIIA p19 subunit
• Gene ID: 2957
• mRNA Refseq: NM_015859
• Protein Refseq: NP_056943
• MIM: 600520
• UniProt ID: P52655
• Chromosome Location: 14q31
• Pathway: Basal transcription factors; Gene Expression; HIV Infection; Transcription
• Function: DNA binding; TATA-binding protein binding; general RNA polymerase II transcription factor activity; protein heterodimerization activity; transcription coactivator activity; transcription factor binding