Recombinant Human CDK2/Cyclin A2, GST-tagged, Active

• Cat.No.: CDK2/CyclinA2-272H
• Product Overview: Recombinant full-length human CDK2 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). CDK2 phosphorylates multiple cellular substrates including SMAD3 and FOXO1. Phosphorylation of FOXO1 leads to its inhibition.
• Molecular Weight: ~58 kDa and CyclinA2 ~78 kDa.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: CDK2 cyclin-dependent kinase 2 [ Homo sapiens ]CCNA2 cyclin A2 [ Homo sapiens ]
• Synonyms: CDK2; cyclin-dependent kinase 2; p33 protein kinase; cell devision kinase 2; cdc2-related protein kinase; EC 2.7.11.22; cell devision protein kinase 2;CCNA2; p33(CDK2); cyclin A2; CCN1; CCNA; Cyclin-A; Cyclin-A2
• Gene ID: 1017890
• mRNA Refseq: NM_001798NM_001237
• Protein Refseq: NP_001789NP_001228
• MIM: 116953123835
• UniProt ID: P24941P20248
• Chromosome Location: 12q134q25-q31
• Pathway: Cell cycle; Pathways in cancer; ; Progesterone-mediated oocyte maturation; Prostate cancer; Small cell lung cancer; p53 signaling pathway
• Function: ATP binding; cyclin-dependent protein kinase activity; histone kinase activity; identical protein binding; nucleotide binding; transferase activity; protein binding