Recombinant Human Cyclin-Dependent Kinase 2, GST-tagged

• Cat.No.: CDK2-999H
• Product Overview: Recombinant full-length human CDK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including Cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). CDK2 phosphorylates multiple cellular substrates including SMAD3 and FOXO1. Phosphorylation of FOXO1 leads to its inhibition.
• Applications: Western Blot
• Molecular Weight: 94 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
• Specific Activity: 31 nmol/min/mg
• Purity: > 80 %
• Concentration: 0.1 ug/ul
• Sequences: Full-length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Activation of ATR in response to replication stress; Activation of the pre-replicative complex; B Cell Receptor Signaling Pathway; BARD1 signaling events; Cell Cycle Checkpoints; Cell Cycle; Cyclin E associated events during G1/S transition; DNA Replication; DNA Replication Pre-Initiation; Delta-Notch Signaling Pathway; E2F transcription factor network; FoxO family signaling; G2/M Checkpoints; Herpes simplex infection; Measles

Gene Information

• Gene Name: CDK2 cyclin-dependent kinase 2 [ Homo sapiens ]
• Official Symbol: CDK2
• Synonyms: CDK2; cyclin-dependent kinase 2; p33 protein kinase; cell devision kinase 2; cdc2-related protein kinase; EC 2.7.11.22; cell devision protein kinase 2; CCNA2; p33(CDK2); cyclin A2; CCN1; CCNA; Cyclin-A; Cyclin-A2
• Gene ID: 1017
• mRNA Refseq: NM_001798
• Protein Refseq: NP_001789
• MIM: 116953
• UniProt ID: P24941
• Chromosome Location: 12q13
• Function: ATP binding; cyclin binding; cyclin-dependent protein kinase activity; contributes_to histone kinase activity; kinase activity; nucleotide binding; protein binding; protein kinase activity