Recombinant Human CDC34 Protein (A2-S236), Tag Free

• Cat.No.: CDC34-0036H
• Product Overview: Recombinant Human (GG)-UB2R1(A2-S236 end) Protein was expressed in Insect cell.

Specification

• Description: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes.
• Source: Insect cell
• Species: Human
• Tag: Non
• Form: Liquid
• Protein length: A2-S236
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 80%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 5% glycerol, 1 mM DTT
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: CDC34 cell division cycle 34 homolog (S. cerevisiae) [ Homo sapiens (human) ]
• Official Symbol: CDC34
• Synonyms: CDC34; cell division cycle 34 homolog (S. cerevisiae); cell division cycle 34; ubiquitin-conjugating enzyme E2 R1; E2 CDC34; UBC3; UBE2R1; ubiquitin carrier protein; ubiquitin-protein ligase R1; ubiquitin-conjugating enzyme E2-CDC34; ubiquitin-conjugating enzyme E2-32 KDA complementing; UBCH3; E2-CDC34
• Gene ID: 997
• mRNA Refseq: NM_004359
• Protein Refseq: NP_004350
• MIM: 116948
• UniProt ID: P49427