Recombinant Human CDC34 293 Cell Lysate

• Cat.No.: CDC34-7660HCL

Specification

• Description: Antigen standard for cell division cycle 34 homolog (S. cerevisiae) (CDC34) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: CDC34 cell division cycle 34 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: CDC34
• Synonyms: CDC34; cell division cycle 34 homolog (S. cerevisiae); cell division cycle 34; ubiquitin-conjugating enzyme E2 R1; E2 CDC34; UBC3; UBE2R1; ubiquitin carrier protein; ubiquitin-protein ligase R1; ubiquitin-conjugating enzyme E2-CDC34; ubiquitin-conjugating enzyme E2-32 KDA complementing; UBCH3; E2-CDC34
• Gene ID: 997
• mRNA Refseq: NM_004359
• Protein Refseq: NP_004350
• MIM: 116948
• UniProt ID: P49427
• Chromosome Location: 19p13.3
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem
• Function: ATP binding; acid-amino acid ligase activity; ligase activity; nucleotide binding; protein binding; ubiquitin-protein ligase activity; ubiquitin-protein ligase activity