Recombinant Human CD4 Molecule (411-459)

• Cat.No.: CD4-104H
• Product Overview: Recombinant Human CD4produced inEscherichia Coli encoding amino acids 411-459.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 411-459 a.a.
• Description: CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. Antibody to CD4 recognizes T-helper cells required for recognition of class II antigens. It reacts with ~60% of peripheral blood E rosette-positive (E+) cells while showing negligible reactivity with E- cells, monocytes, granulocytes, EBV-transformed B cell lines, and mouse splenocytes.
• Physical Appearance: Sterile filtered liquid.
• Formulation: Recombinant Human CD4 protein at 100µg/ml in 50mM Tris-Acetate, pH-7.5, 1mM EDTA and 20% Glycerol.
• Application and Suggested Dilutions: •ELISA •Inhibition Assays •Western Blotting
• Characterization: On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 48kDa.
• Storage: Store vial at -20°Cto -80°C. When stored at the recommended temperature, this protein is stable for 12 months. Please avoid freeze-thaw cycles.

Gene Information

• Gene Name: CD4 molecule [ Homo sapiens ]
• Synonyms: Gp55; HLA-2; L3 / T4; Ly-4; T cell antigen T4/LEU3; T4; sCD4; CD4mut; CD4; CD4 antigen; CD4 antigen (p55); CD4 molecule; CD4 receptor; T-cell surface antigen T4/Leu-3; CD4 molecule; 12pter-p12
• Gene ID: 920
• mRNA Refseq: NM_000616
• Protein Refseq: NP_000607
• MIM: 186940
• UniProt ID: P01730
• Chromosome Location: 12pter-p12
• Pathway: Primary immunodeficiency; Antigen processing and presentation; Hematopoietic cell lineage; Cell adhesion molecules (CAMs); T cell receptor signaling pathway
• Function: MHC class II protein binding; coreceptor activity; extracellular matrix structural constituent; glycoprotein binding; protein kinase binding; zinc ion binding; protein homodimerization activity; transmembrane receptor activity