Active Recombinant Human CD3E & CD3G Protein, FLAG-Fc & mFc-tagged
Cat.No. : | CD3E & CD3G-761H |
Product Overview : | A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (NP_000724.1) was fused with the C-terminal FLAG-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Ser116) of human CD3G (NP_000064.1) was fused the Fc region of mouse IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3E/CD3G heterodimer was purified. |
- Specification
- Gene Information
- Related Products
- Download
Species : | Human |
Source : | HEK293 |
Tag : | Fc&Flag&mFc |
Protein Length : | Met1-Asp126 & Met1-Ser116 |
Form : | Lyophilized from sterile PBS, pH 7.4 |
Bio-activity : | Measured by its ability to bind OKT3-mIgG2a in a functional ELISA. |
Molecular Mass : | The recombinant heterodimer of human CD3E/CD3G comprises 681 (353+328) amino acids and has a calculated molecular mass of 76.9 (39.8+37.1) KDa. As a result of glycosylation, the apparent molecular mass of human CD3E/CD3G heterodimer is approximately 42-47 KDa in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 95 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
◆ Recombinant Proteins | ||
CD3E&CD3G-348R | Recombinant Rhesus CD3E&CD3G protein, hFc-tagged | +Inquiry |
CD3E & CD3G-761H | Active Recombinant Human CD3E & CD3G Protein, FLAG-Fc & mFc-tagged | +Inquiry |
CD3E & CD3G-3034M | Active Recombinant Mouse CD3E & CD3G protein, Fc-His &Fc-Flag-tagged | +Inquiry |
CD3E & CD3G-34H | Active Recombinant Human CD3E & CD3G protein, His-Avi-tagged, Biotinylated | +Inquiry |
CD3E & CD3G-146H | Recombinant Human CD3E & CD3G protein, Fc-tagged | +Inquiry |
◆ Cell & Tissue Lysates | ||
CD3E & CD3G-761HCL | Recombinant Human CD3E & CD3G cell lysate | +Inquiry |
Not For Human Consumption!
Inquiry
- Reviews
- Q&As
Ask a Question for All CD3E;CD3G Products
Required fields are marked with *
My Review for All CD3E;CD3G Products
Required fields are marked with *
0
Inquiry Basket