Recombinant Human CD3E&CD3D protein, hFc-Avi-tagged, Biotinylated

• Cat.No.: CD3E & CD3D-378H
• Product Overview: Biotinylated Recombinant Human CD3E&CD3D protein(P07766(CD3E)&P04234(CD3D))(Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D)), fused with C-terminal hFc-Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: C-hFc-Avi
• ProteinLength: Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D)
• Tag: C-hFc-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human CD3E&CD3D, hFc Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 42.1ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 40 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• GeneID 2: 915

Gene Information

• Gene Name: CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ]
• Official Symbol: CD3E
• Synonyms: CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683
• Gene ID: 916
• mRNA Refseq: NM_000733
• Protein Refseq: NP_000724
• UniProt ID: P07766
• Gene Name 2: CD3D CD3d molecule, delta (CD3-TCR complex) [ Homo sapiens ]
• Official Symbol 2: CD3D
• Synonyms 2: CD3D; CD3d molecule, delta (CD3-TCR complex); CD3d antigen, delta polypeptide (TiT3 complex) , T3D; T-cell surface glycoprotein CD3 delta chain; CD3 delta; OKT3, delta chain; CD3 antigen, delta subunit; T-cell receptor T3 delta chain; CD3d antigen, delta polypeptide (TiT3 complex); T3D; CD3-DELTA
• mRNA Refseq 2: NM_000732
• Protein Refseq 2: NP_000723
• MIM 2: 186790
• UniProt ID 2: P04234