Active Recombinant Human CD3E&CD3D Protein, Fc-tagged

• Cat.No.: CD3E&CD3D-3617H
• Product Overview: The recombinant human CD3e-CD3d heterodimer is co-expressed as 2 chains: (1) a 326-amino acid CD3e-Fc chain consisting of Gln22 – Val118 region of CD3e and a C-terminal Fc from human IgG1; (2) a 317-amino acid CD3d-Fc fusion consisting of Phe22 - Met92 region of CD3d and a C- terminal Fc from human IgG1. It exists as a dimer of heterodimer under non-reducing condition.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 22-118;22-92 a.a.
• Form: Liquid
• Bio-activity: Recognized by recombinant anti-CD3e monoclonal antibodies: human IgG1, mouse IgG1, mouse IgG2a and rabbit IgG and other OKT3 derived antibodies that binds CD3e in the context of a CD3e:CD3d complex.
• Molecular Mass: Calculated molecular mass (kDa): 37; Estimated by SDS-PAGE under reducing condition (kDa): ~45
• AA Sequence: chain1 (CD3e-Fc): QDGNEEMGGI TQTPYKVSIS GTTVILTCPQ YPGSEILWQH NDKNIGGDED DKNIGSDEDH LSLKEFSELE QSGYYVCYPR GSKPEDANFY LYLRARVSGD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSREEMTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK
  chain2 (CD3d-Fc): FKIPIEELED RVFVNCNTSI TWVEGTVGTL LSDITRLDLG KRILDPRGIY RCNGTDIYKD KESTVQVHYR MSGDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK GGGLNDIFEA QKIEWHE
• Endotoxin: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
• Purity: > 95%
• Storage: It is recommended to store small aliquots at the temperature below -20 centigrade for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4 centigrade for no more than 2 weeks. Avoid repeated freeze/thaw cycles.
• Storage Buffer: Sterile PBS pH7.4

Gene Information

• Gene Name: CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ]
• Official Symbol: CD3E
• Synonyms: CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683
• Gene ID: 916
• mRNA Refseq: NM_000733
• Protein Refseq: NP_000724
• UniProt ID: P07766