Recombinant Human CD33 Protein, His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: CD33-234HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human CD33 (AAH28152.1) (Met1-His259), fused with a C-terminal polyhistidine tag, was produced in Human Cells.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized
• Molecular Mass: The recombinant human CD33 comprises 253 amino acids and has a predicted molecular mass of 28.2 kDa. The apparent molecular mass of the protein is approximately 40-46 kDa in SDS-PAGE under reducing conditions.
• Protein length: 253
• N-terminal Sequence Analysis: Asp 18
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4, 10% glycerol. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CD33 CD33 molecule [ Homo sapiens ]
• Official Symbol: CD33
• Gene ID: 945
• mRNA Refseq: NM_001082618
• Protein Refseq: NP_001076087
• MIM: 159590
• UniProt ID: P20138