Active Recombinant Rat CD33 protein, His-tagged

• Cat.No.: CD33-0705R
• Product Overview: Recombinant Rat CD33 protein(Asp18-Gln240), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Rat
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Rat Siglec-3, His Tag at 1 μg/mL (100 μL/well) can bind Anti-CD33 antibody, Mouse IgG1 with a linear range of 0.2-16 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 26.7 kDa. The protein migrates as 37-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Asp18-Gln240
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 18-240 a.a.