Recombinant Human CCS protein, His-tagged

• Cat.No.: CCS-1053H
• Product Overview: Recombinant Human CCS protein(Gln23~Gln269), fused with N-terminal His tag, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Gln23~Gln269
• Form: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Bio-activity: Superoxide dismutase copper chaperone (SOD4) is an enzyme that in humans is encoded by the CCS gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. Acroding to the report, in a weakly alkaline buffer solution (pH=8.2) with N-tris(hydroxymethyl) amino methane-HCL, pyrogallol can occur autoxidation in the air, then SOD can inhibit this reaction. Thus, we use this way to measued the activity of recombinant human SOD4. The reaction was performed in adding 5 μl 5 mmol/L pyrogallol to 200 μl 50mmol/L Tris-HCl, rapidly mixing at 25°C, then read at 325 nm (using 50 mmol/L Tris-HCl as blank control) in kinetic mode for 3 minutes using a microplate reader controlling the pyrogallol autoxidation rate at 0.70 OD/min. Different concentrations of recombinant human SOD4 were added into 200 μl 50 mmol/L Tris-HCl, incubated for 20min at 25°C, then adding 5 μl 5 mmol/L pyrogallol to each well, rapidly mixing and read at 325 nm in kinetic mode for 3 minutes. Under these conditions, the enzyme amount of 50% inhibition of pyrogallol autooxidation per minute is defined as a unit. The specific activity of recombinant human SOD4 is 97 U/mg.
• Molecular Mass: 29.9kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: >95%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: CCS copper chaperone for superoxide dismutase [ Homo sapiens ]
• Official Symbol: CCS
• Synonyms: CCS; copper chaperone for superoxide dismutase; superoxide dismutase copper chaperone; MGC138260
• Gene ID: 9973
• mRNA Refseq: NM_005125
• Protein Refseq: NP_005116
• MIM: 603864
• UniProt ID: O14618