Recombinant Human CC2D1A 293 Cell Lysate

• Cat.No.: CC2D1A-7799HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for coiled-coil and C2 domain containing 1A (CC2D1A) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: CC2D1A coiled-coil and C2 domain containing 1A [ Homo sapiens ]
• Official Symbol: CC2D1A
• Synonyms: CC2D1A; coiled-coil and C2 domain containing 1A; coiled-coil and C2 domain-containing protein 1A; FLJ20241; mental retardation; nonsyndromic; autosomal recessive; 3; MRT3; Akt kinase-interacting protein 1; putative NFkB activating protein; FRE under dual repression-binding protein 1; putative NF-kappa-B-activating protein 023N; mental retardation, nonsyndromic, autosomal recessive, 3; five repressor element under dual repression-binding protein 1; five prime repressor element under dual repression-binding protein 1; FREUD-1; Freud-1/Aki1; FLJ41160
• Gene ID: 54862
• mRNA Refseq: NM_017721
• Protein Refseq: NP_060191
• MIM: 610055
• UniProt ID: Q6P1N0
• Chromosome Location: 19p13.12
• Pathway: SIDS Susceptibility Pathways, organism-specific biosystem
• Function: DNA binding; signal transducer activity