Recombinant Human CBFA2T2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: CBFA2T2-5030H
• Product Overview: CBFA2T2 MS Standard C13 and N15-labeled recombinant protein (NP_001034798) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 68 kDa
• AA Sequence: MGFHHVGQARLELLTSGDLPALASQRAGITVGPEKRVPAMPGSPVEVKIQSRSSPPTMPPLPPINPGGPRPVSFTPTALSNGINHSPPTLNGAPSPPQRFSNGPASSTSSALTNQQLPATCGARQLSKLKRFLTTLQQFGNDISPEIGEKVRTLVLALVNSTVTIEEFHCKLQEATNFPLRPFVIPFLKANLPLLQRELLHCARAAKQTPSQYLAQHEHLLLNTSIASPADSSELLMEVHGNGKRPSPERREENSFDRDTIAPEPPAKRVCTISPAPRHSPALTVPLMNPGGQFHPTPPPLQHYTLEDIATSHLYREPNKMLEHREVRDRHHSLGLNGGYQDELVDHRLTEREWADEWKHLDHALNCIMEMVEKTRRSMAVLRRCQESDREELNYWKRRYNENTELRKTGTELVSRQHSPGSADSLSNDSQREFNSRPGTGYVPVEFWKKTEEAVNKVKIQAMSEVQKAVAEAEQKAFEVIATERARMEQTIADVKRQAAEDAFLVINEQEESTENCWNCGRKASETCSGCNIARYCGSFCQHKDWERHHRLCGQNLHGQSPHGQGRPLLPVGRGSSARSADCSVPSPALDKTSATTSRSSTPASVTAIDTNGLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: CBFA2T2 CBFA2/RUNX1 partner transcriptional co-repressor 2 [ Homo sapiens (human) ]
• Official Symbol: CBFA2T2
• Synonyms: CBFA2T2; core-binding factor, runt domain, alpha subunit 2; translocated to, 2; protein CBFA2T2; MTGR1; ZMYND3; MTG8-like protein; MTG8-related protein 1; ETO homolog on chromosome 20; ETO homologous on chromosome 20; myeloid translocation-related protein 1; myeloid translocation gene-related protein 1; EHT; p85; DKFZp313F2116
• Gene ID: 9139
• mRNA Refseq: NM_001039709
• Protein Refseq: NP_001034798
• MIM: 603672
• UniProt ID: O43439