Recombinant Human casein kinase 2, beta polypeptide

• Cat.No.: CSNK2B-23H
• Product Overview: Recombinant Human Casein Kinase 2 beta is a non-glycosylated polypeptide having a molecular mass of 24.9 kDa. Casein Kinase 2 is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: CSNK2B-23H
• Description: The Casein kinase 2 (EC 2.7.11.1) is a serine/threonine-selective protein kinase that is a tetramer of two alpha subunits and two beta subunits. The alpha subunits have the catalytic kinase domain. Casein kinase 2 has been implicated in cell cycle control, DNA repair, regulation of the circadian rhythm and other cellular processes. Casein kinase 2 activity has been reported to be activated following Wnt signaling pathway activation. A Pertussis toxin-sensitive G protein and Disheveled appear to be an intermediary between Wnt-mediated activation of the Frizzled receptor and activation of casein kinase 2.
• Source: Escherichia Coli.
• Unit Definition: 1U = 1µmol/min at 37 degree C) using the synthetic peptide RRRDDDSDDD.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 95% as determined by SDS-PAGE.
• Formulation: CK2 lyophilized from 25mM Tris-HCl, 500mM NaCl, 1mM DTT, 1mM EDTA, protease inhibitor cocktail, pH 8.5.
• Solubility: Solubilize in distilled H₂O or 50% glycerol.
• Biological Activity: Specific activity > 1U/mg.
• Storage: Lyophilized CK2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CK2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
• Tag: Non

Gene Information

• Gene Name: CSNK2B casein kinase 2, beta polypeptide [ Homo sapiens ]
• Synonyms: CSNK2B; casein kinase 2, beta polypeptide; G5A; CK2B; CK2N; MGC138222; MGC138224; phosvitin; OTTHUMP00000029063; OTTHUMP00000029064; Casein kinase II beta subunit; alternative name: G5a, phosvitin; CSK2B; OTTHUMP00000029060; Phosvitin
• Gene ID: 1460
• mRNA Refseq: NM_001320
• Protein Refseq: NP_001311
• MIM: 115441
• UniProt ID: P67870
• Chromosome Location: 6p21-p12; 6p21.3
• Pathway: Adherens junction; Tight junction; Wnt signaling pathway
• Function: identical protein binding; protein domain specific binding; protein kinase regulator activity; protein serine/threonine kinase activity