Recombinant Human CA4 protein, His-tagged

• Cat.No.: CA4-7835H
• Product Overview: Recombinant Human CA4 aa. (Ala19~Lys283 (Accession # P22748)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Ala19~Lys283
• Description: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, it may have a role in inherited renal abnormalities of bicarbonate transport.
• Form: Freeze-dried powder
• Molecular Mass: 32 kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.5.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: CA4 carbonic anhydrase 4 [ Homo sapiens (human) ]
• Official Symbol: CA4
• Synonyms: CA4; carbonic anhydrase 4; CAIV; Car4; RP17; CA-IV; carbonate dehydratase IV; carbonic anhydrase IV; carbonic dehydratase IV
• Gene ID: 762
• mRNA Refseq: NM_000717.4
• Protein Refseq: NP_000708.1
• UniProt ID: P22748